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Cell-free experiments demonstrate that translocation of secretory proteins into

ID: 74827 • Letter: C

Question

Cell-free experiments demonstrate that translocation of secretory proteins into microsomes (as experimental substitute for the [____]) is coupled to translation. In an in vitro protein synthesis system, to which an mRNA encoding a secretory protein is added, the secretory protein [____] in the absence of microsomes, but it is translocated across the vesicle membrane and is incorporated into these microsomes in a [____] form only if [____] are present during protein synthesis.

Please pick one.

(1) rough ER; is not synthesized; extended; presequences

(2) endoplasmic reticulum; is synthesized; protease-resistant; microsomes

(3) smooth ER; disappears; detergent-resistant; signal presequences

(4) signal recognition system; is proteolyzed; intact; SRP recognition signals

(5) polyribosomes; is not proteolyzed; detergent and protease; SRPs

Explanation / Answer

Answer is 1

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