Cell-free experiments demonstrate that translocation of secretory proteins into
ID: 74827 • Letter: C
Question
Cell-free experiments demonstrate that translocation of secretory proteins into microsomes (as experimental substitute for the [____]) is coupled to translation. In an in vitro protein synthesis system, to which an mRNA encoding a secretory protein is added, the secretory protein [____] in the absence of microsomes, but it is translocated across the vesicle membrane and is incorporated into these microsomes in a [____] form only if [____] are present during protein synthesis.
Please pick one.
(1) rough ER; is not synthesized; extended; presequences
(2) endoplasmic reticulum; is synthesized; protease-resistant; microsomes
(3) smooth ER; disappears; detergent-resistant; signal presequences
(4) signal recognition system; is proteolyzed; intact; SRP recognition signals
(5) polyribosomes; is not proteolyzed; detergent and protease; SRPs
Explanation / Answer
Answer is 1
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