You have cloned the cDNA of a pump protein from human stomach cells which you th

Question

You have cloned the cDNA of a pump protein from human stomach cells which you think is a passive K^+ channel which can pump K^+ from inside the cell to the extra cellular space. To generate experimental evidence for this, you design the following experiment: you ligate the cDNA coding the K^+ pump into an expression vector. You then transfect frog oocytes with this plasmid. You then put your transfected oocytes into the 3 different solutions shown below. What will happen to these cells if you put them into these following solutions? Please write your expected results for each solution: (cytoplasmic concentrations of the following ions in oocytes are: K^+: 150mM, Na^+: 50mM; Cl^-: 10mM). Solution A (20mM KCl): Solution C (550 mM KCl): Solution B (50mM NaCl): Why did you use frog oocytes for these experiments?:

Explanation / Answer

In the first case, the concentration of kcl is more Inside the oocytes than outside therefore it is hypotonic solution, the cell will shrink.

In second case, the concentration of kcl is more outside the oocytes than inside therefore it is a hypertonic solution the cell will expand.

In third case, the concentration remains same,outside as well inside the oocytes therefore it is isotonic solution, the cell will not shrink and not expand, remain same in concentration.

In fourth case, frog oocytes is used it has semipermeable membrane without which the theory of passive transport can't be proven.

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