(d) large assemblies of subunits Question 13. In two mutagenesis experiments, on
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(d) large assemblies of subunits Question 13. In two mutagenesis experiments, one in which saturation mutagenesis was used with EMs and the other where X-rays were used, eight mutations for the dumpy phenotype were recovered in C elegans. Complementation analysis was done with all 8 strains to determine the number of genes identified in both experiments. The data are as follows: means wild type phenotype, means mutant phenotype, 0 means no adult worms were obtained How many genes are identified? Which deletions do these genes map to?Explanation / Answer
Please note:
According to first column, A, B, E, F, G, and H complement each other. So, they are different genes.
A, C and D are same.
2nd column: B, D, E, F, G, H complement each other. So, they are different.
B and C are same. They do not complement each other.
3rd column: C, E, F, G, H are different. They complement each other.
C and D are same.
4th column: D, E, F, G, H complement each other.
5th column: E, F, and G do not complement each other. They are different genes.
6th column: F, G, and H do not complement each other.
7th column: G and H do not complement each other.
So, the number of same genes are A, B, C, D, E, F, G and H.
This finally means that there is only one gene here.
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