A carbonic anhydrase generates H+ at a rate constant (K3) of 10^(6) per second.

Question

A carbonic anhydrase generates H+ at a rate constant (K3) of 10^(6) per second.  Assume the rate constant of the enzyme is not affected by pH, and the initial pH of the reaction solution = 7.0.  


1) What will be the pH in the reaction solution after 0.1 nM carbonic anhydrase was incubated with unlimited substrate (CO2) for 1 min?



2) What will be the pH in the above reaction solution (0.1 nM carbonic anhydrase incubated with unlimited substrate (CO2) for 1 min) if 100 mM Tris (pKa = 8.0) was included as buffer (initial pH = 7.0)?

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