Use the given information within the Fecal Lab document as well as outside resou

Question

Use the given information within the Fecal Lab document as well as outside resources to solve the following case scenarios by identification of the probable gram negative fecal pathogen. The object is to try to identify the bacterium down to the Genus species level if possible. More than one organism may be responsible.

A female patient had entered the emergency room at the local hospital complaining about GI distress, nausea and diarrhea. A fecal sample taken to the medical testing lab was routinely tested according to the diagnostic outline for enteric pathogens. Enrichment and isolation was carried out on Hektoen and MacConkey agar with the following results: Clear, colorless colonies of different sizes onMacConkey agar l Greenish-blue colonies of different sizes on Hektoen agar. Two different sizes of bacilli that are both gram negative Both sizes of colonies wereconsidered to be two different potential enteric pathogens and were used to inoculate a set of Kligler Iron agar slants Urea broth and motility with the following results Kligler Iron Agar Motility Agar Urea Broth

Explanation / Answer

Sample 1 might be Salmonella typhi. It is H2S positive, motile and Urease negative.

Sample 1 cannot be identified definitively as multiple organisms share the same properties.

Citrobacter freundii, Proteus mirabilis and Proteus vulgaris, Morganella and Providencia have similar properties.

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