Cell cycle d. When researchers inject cytoplasm from an M phase cell into an int

Question

Cell cycle

d. When researchers inject cytoplasm from an M phase cell into an interphase cell, the cell will enter M phase. What two proteins are found in the M phase cytoplasm that cause the interphase cell to enter M phase?

e. The levels of which of the two proteins changes during the cell cycle?

f. Will levels of that protein be higher during M phase or interphase?

g. The activity of which of the two proteins is regulated by phosphorylation?

h. Describe how phosphorylation affects the activity of that protein.

Explanation / Answer

d. The two proteins that are found in the M phase cytoplasm that cause the interphase cell to enter M phase are Cyclins and Cyclin-dependent Kinases.

e. levels of the two proteins that changes during the cell cycle are: Cyclins and Cyclin-dependent Kinases

f. The levels of these proteins will be higher during M phase

g. The activity of CDK is regulated by Phosphorylation.

h. Phosphorylation affects the activity of CDK as the activation of this protein requires binding with cyclin and phosphorylation of the threonine residue 160 by enzyme CAK. The phosphorylated CDKs control transcription, mRNA processing.

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