How would you determine whether a colorless colony on MacConkey agar is Salmonel
ID: 79171 • Letter: H
Question
How would you determine whether a colorless colony on MacConkey agar is Salmonella or Shigella? Why would you want to identify a colorless colony? Tests to determine the presence of Bacteroides in clams and oysters are being developed. If these bacteria do not present a health hazard, why are these tests being developed? You are called to investigate an outbreak of diarrheal disease in a child care center. The symptoms include vomiting, fever, nausea, and cramps. You culture fecal samples from children and find a gram-negative, lactose-negative rod. The bacterium does not produce gas from glucose and makes colorless colonies on MacConkey agar. Use Appendix to identify the genus of this bacterium.Explanation / Answer
ANSWER:
1) Salmonella has Lysine DeCarboxylase, whereas Shigella does not. so, colorless colony in Macconkey Agar is Salmonella.
2) Oysters on the half shell lose their glamour if they've got Campylobacter or Salmonella lurking in their tissues. These bacterial pathogens are leading causes of food-borne illness around the world and are a particular concern in raw or lightly cooked shellfish..the prevalence of Campylobacter and Salmonella causes food poisoning . the possibility of resistance gene transfer between microbes, due to fecal bacteria, within the oysters. Transconjugant colonies were recovered from the bodies of experimental oysters, and verified via growth on selective media and PCR amplification . they causess food poisoning in humans.so It is important to identify organisms to eliminate them as the agent for the disease.
3)The genus of this bacterium is Shigella species.
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