you have an oliginucleotides probe that hybridizes to part of Gene A from a euka
ID: 79192 • Letter: Y
Question
you have an oliginucleotides probe that hybridizes to part of Gene A from a eukaryotic cell. Indicate whether a cdna library or a genomic DNA Library will be more appropriate for the use of the following applications.
A. you want to study the promoter of a gene A.
B. Gene A encodes a tRNA and you wish to isolate a piece of DNA containing the full-length sequence of the tRNA.
C. you discover that gene A is alternatively spliced and you want to see which predicted alternative splice products of the cell actually produces.
D. you want to find both gene A in the genes located near gene A on the chromosome.
E. you want to express Gene A in bacteria to produce lots of protein A.
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Explanation / Answer
A probe is an oligonucleotide that is designed to be complementary to the mRNA of interest so that it can be used to screen a library, any mRNA produces a unique polypeptide when it is translated but not vice versa , triplet code is degenerated thar are many mRNA sequence might produce the same sequence of amino acid.
The effect of force expression of any gene in any tissue of interest. By taking a advantage of understanding a particular promoter element tht are required for the expression of a protein in a particular tissue at a particular time. it is possible to make gene or ahybrid gene tht expresses any protein of interest possible to determine the effect.
A: Genomic library cDNAs are produced from mRNAs so thr promoters will not be included in cDNA library.
B: cDNAs are generally constructed by using an oligo dT primer, tRNAs do not have PolyA Tails cDNA library were made using random primers contain full length version of tRNAs
C: cDNA library: Isolating cDNA would tell you which splice variant are produced ina cell.
D: Genomic DNA fragment can contain the gene next to your gene of interest: cDNA will not
E: Genomic library: Bacteria do not have ability to remove Introns which may exist DNA extracted from gene library.
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