you have accidently torrent labels off two tubes, each containing a different pl

ID: 79193 • Letter: Y

Question

you have accidently torrent labels off two tubes, each containing a different plasmid, and now do not know which plasmin is in which two. Fortunately, you have restriction maps for both plasmids, show and figure Q 10-6. You have the opportunity to test just one sample from one of your tubes. You have equipment for agarose gel electrophoresis, and standard set of DNA size markers, and the necessary restriction enzymes. Fill in the following table and determine which restriction enzyme digests would help you identify the samples.

I'm not sure how to do this problem help please. thank you

Fill in the following table BamHI ident Ecoai 05 kb 0.3 kb plasmid A 2 kb llamHI 0.4 kb EcoRI BamHl 0.2 k 0.3 kb 0 kb 05 kb plasmid B Barnhill 0.4 kb Hrvdill 100 kb CORI

Explanation / Answer

Restriction enzymes are used to digest the DNA by nicking DNA molecules at the positions at which specific short sequences of bases are present.

Yes : Plasmid can be Identify by given particular restriction enzyme.

No: Plasmid cannot be Identify by given particular restriction enzyme.

Plasmid A

Plasmid B

Eco R1

Yes

BamH1

Yes

BgIII

Yes

HindIII

Yes

EcoRI + BamHI

Yes

EcoRI + BgIII

Yes

EcoRI + HidIII

Yes

BamHI + BgIII

Yes

BamHII+ HindIII

None

BgIII + HindIII

Yes