Two compounds (A) and (B) with distribution coefficients of 15 and 18 respective

ID: 799344 • Letter: T

Question

Two compounds (A) and (B) with distribution coefficients of 15 and 18 respectively are

separated on a column with where the volumes of the stationary and mobile phases are 1.0 and 3.0 mL

respectively. The retention time for an unretained species was found to be 1.0 minutes.

{A} Calculate the retention times for the two compounds (A) and (B).

{B} If the peak widths for the two compounds were both 0.80 minutes, calculate the resolution for the

separation under these conditions.

{C} What are the number of theoretical plates based on Compound A under these conditions?

{D} What would N have to be (based on Compound A) in order to improve the resolution to 2.0.

Assume that only the peak widths change and the retention times do not change?

Two compounds (A) and (B) with distribution coefficients of 15 and 18 respective

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