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Which of the following changes would improve a chromatographic separation? (eith

ID: 800817 • Letter: W

Question

                    Which of the following changes would improve a chromatographic separation? (either GC or LC-type). Circle all that apply. For full credit, write a short phrase that explains WHY the separation would be improved or degraded.                 

                    (a) decreasing the average particle size of the stationary phase (support material)                 

                    (why?)                 

                    (b) increasing the uniformity of size for the particles of the stationary phase support material                 

                    (why?)                 

                    (c) decreasing the thickness of any coatings on stationary phase support material                 

                    (why?)                 

                    (d) decreasing variation in coating thickness, of coatings on the stationary phase support material                 

                    (why?)                 

                    (e) increasing temperature of the column during a GC experiment                 

                    (why?)                 

                    (f) increasing the polarity of mobile phase in a reverse-phase HPLC                     separation.                 

                    (why?)                 

Explanation / Answer

(c) decreasing the thickness of any coatings on stationary phase support material

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