The molecular weight of vincristine, a pharmaceutical drug, is 923.04 g/mol. It

Question

The molecular weight of vincristine, a pharmaceutical drug, is 923.04 g/mol. It is supplied in 1 mg per vial. You want to treat your culture cells with a final concentration of 7 hM vincristine and will need 12 mL of cells for your experiment. You only want to add 21 mL of vincristine solution to your cells. What concentration should you make your stock solution of vincristine in order to make this 21 uL addition?The molecular weight of vincristine, a pharmaceutical drug, is 923.04 g/mol. It is supplied in 1 mg per vial. You want to treat your culture cells with a final concentration of 7 hM vincristine and will need 12 mL of cells for your experiment. You only want to add 21 mL of vincristine solution to your cells. What concentration should you make your stock solution of vincristine in order to make this 21 uL addition?

Explanation / Answer

Calculate the concentration of stock solution of Vincristine:

M1V1=M2V2

Here, M1=?, V1=30 mL, M2= 5 nM or 5*10-6 mM, V2= 1uL or 0.001 mL

M1*30=5*10-6*0.001

M1=5*10-6*0.001/30

=1.667-10-10 mM

=1.667-10-10 mM of solution is needed to prepare 21 uL of stock solution.

Related Questions

Navigate

• Browse (All)
• Browse T
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.