Could you explain why? Thank you. Several E. coli mutants were isolated. beta-Ga

Question

Could you explain why? Thank you.

Several E. coli mutants were isolated. beta-Gactosidase activity was measured for each mutant alone and for the various combinations shown. The table below shows the activity for each strain when grown on the indicated medium. Glycerol is considered to be both a non-inducing and non-repressing carbon source. Mutant 3 was determined to be frameshift mutation in the lacZ coding region and thus has no beta-gactosidase|activity under any condition. Which of the remaining mutants could be mutation in the DNA sequence that CRP binds to? A. Mutant 1 B. Mutant 2 C. both mutants 1 or 2 D. neither mutants 1 or 2

Explanation / Answer

Choice C: both mutant 1 or 2 is correct as CRP as CRP-cAMP has been shown to activate transcription initiation at the Escherichia coli lac promoter and according to the data beta-galactosidase activity is not seen both the cases.

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