4. Consider the DNA sequence below: 3’ – T A C C A A G G A G C A T T A G A T A C

Question

4. Consider the DNA sequence below:

3’ – T A C C A A G G A G C A T T A G A T A C T – 5’

5’ – A T G G T T C C T C G T A A T C T A T G A – 3’

a. Write the mRNA sequence that would be produced if the TOP DNA strand were used as the template strand (label the 5’ and 3’ ends):

b. What is the amino acid sequence of the protein that would be made using the mRNA above in part a ?

c. Write the mRNA sequence that would be produced if the BOTTOM DNA strand were used as the template strand :

d. What is the amino acid sequence of the protein that would be made using the mRNA above in part c ?

e. What is the amino acid sequence of the protein that would be made using the mRNA above in part c ?

Explanation / Answer

mRNA is always from 5'->3' and same as the sense strand or template strand except change T of DNA into U for RNA

DNA sequence:

3’ – T A C C A A G G A G C A T T A G A T A C T – 5’

A) mRNA sequence:

5' - U C A U A G A U U A C G A G G A A C C A U - 3'

B) Amino acid sequence:

Ser - stop

As ,UAG is a stop codon


DNA sequence:
5’ – A T G G T T C C T C G T A A T C T A T G A – 3’

C) mRNA sequence:

5'- A U G G U U C C U C G U A A U C U A U G A - 3'

D) Amino acid sequence :

Met Val Pro Arg Asn Leu stop
UGA is a stop codon

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