8. Examine the table of recognition sites for restriction enzymes below. Cutting

Question

8. Examine the table of recognition sites for restriction enzymes below. Cutting with which two restriction enzymes would generate fully complementary sticky ends that could be used to construct recombinant DNA?

Enzyme Recognition/Cleavage Site:

BamHI G|GATCC

EcoRI G|AATTC

PstI CTGCA|G

BglII A|GATCT

BasI TGATC|A

EesIII C|CTAGG

a. BamHI and EcoRI

b. BamHI and PstI

c. BamHI and BglII --> correct answer

d. BamHI and BasI

e. BamHI and EesIII

Can you please explain to me why this is the answer? Thank you!

Explanation / Answer

a. BamHI and EcoRI
G GATCC
CCTAG G

G AATTC
CAATT G

GATCC FROM BAM HI
CAATT FROM ECO RI - NO COMPLEMENTARITY - NO USE IN construct OF recombinant DNA

b. BamHI and PstI
G GATCC
CCTAG G

CTGCA G
G ACGTC

GATCC FROM BAM HI
CTGCA FROM PST I - NO COMPLEMENTATION- NO USE IN construct OF recombinant DNA

c. BamHI and BglII
G GATCC
CCTAG G

A GATCT
TCTAG A

GATCC FROM BAM HI'
TCTAG FROM BGLII - COMPLEMENTATION IS THERE

d. BamHI and BasI
G| GATCC
CCTAG| G

TGATC| A
A| CTAGT

GATCC ROM BAM HI'
TGATC FROM BAS I - NO COMPLEMENTATION- NO USE IN construct OF recombinant DNA

e. BamHI and EesIII
G| GATCC
CCTAG| G

C| CTAGG
GGATC| C

GATCC FROM BAMHI
GGATC FROM EES III - NO COMPLEMENTATION - NO USE IN construct OF recombinant DNA

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