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1) The reaction for converting 2-phosphoglycerate to phosphoenolpyruvate as carr

ID: 820612 • Letter: 1

Question

1) The reaction for converting 2-phosphoglycerate to phosphoenolpyruvate as carried out by the enzyme enolase is shown in the second line.

a) What kind of reaction overall is this?

B) What kind of reaction is the 1st step in getting to the enolic intermediate? What role does Lys345 play in this step?

c) What kind of reaction is the 2nd step in the reaction (getting to phophoenolpyryvate)? What role does Glu121 play in this step?

d) What role do the Mg2+ ions play in this reaction?

Bulleted List 2 - phospoglyoerate boind to enzyme Enolic intermediate phospoenolpyruvate

Explanation / Answer

a) E1cB elimination reaction

b) Enolate formation. Enolase

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1) The reaction shown below is an example of an E2 elimination (from Chem 301A).

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