Human serum albumin (HSA) has a molecular weight of 66,000 g/mol. One compartmen

Question

Human serum albumin (HSA) has a molecular weight of 66,000 g/mol. One compartment contains a solution with HSA and the other compartment in the osmotic cell contains only an electrolyte solution. At a pH of 8 the net charge on HSA is -20. (a) The compartment that only has the electrolyte solution is maintained at a pH = 8 with 1 mu M Solution of NaOH. Find the concentration of Na+ and OH- in the HSA-containing compartment with a protein concentration of 40 mg/g-water. (b) What is the osmotic pressure at 298.15K in the cell in part (a)? (c) Compute the electrostatic potential difference between the two cells in part (a).

Explanation / Answer

both compartments have the same pH and due to semi-permeable nature of the membrane, no ion will move to the other compartment.

the osmotic pressure (P) = CRT. where C= molar concentration, R= ideal gas constant (0.0821 L atm / mol K) and

T= temp in Kelvin.

P= 606 x 10-6 x 0.0821 x 298.15 = 14.83 x 10-3

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