Your superb PhD project has earned you a post-doctoral position in a top lab dev

Question

Your superb PhD project has earned you a post-doctoral position in a top lab developing puffer fish as a system for studying gene organization. Your goal is to find a puffer fish homologue of a human oncogene (a gene that causes cancer). Produce a brief outline for such a project, from checking for homologous sequences in the puffer fish genome, to isolating a puffer fish cDNA for this gene.

Explanation / Answer

We isolate the human oncogene--->sequence it--->isolate genome of puffer fish--->annealing of puffer fish genome to separate the DNA strands--->hybridize with human DNA--->the homologous strand will hybridize with human DNA--->DNA sequence of complementary strand of puffer fish noted--->mRNA isolated from the fish with help of DNA--->cDNA constructed--->5' and 3' RACE[rapid amplification of cDNA ends] done---->they are used to find out full length cDNA of target gene without constructing a cDNA library.

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