Given the following DNA coding sequence (the spaces are guides for the eye ONLY)

Question

Given the following DNA coding sequence (the spaces are guides for the eye ONLY) 3'- ATC AGT ATG ATG TGA CGT CAT AGC GTA TTG TCA G -5' What is the corresponding RNA sequence (indicate the 5' and 3' ends)? What is the one-character (from the table) polypeptide sequence for the protein coded for by the above RNA sequence (including any start or stop codons)? An insertion (underlined) resulted in the following mutation of the coding strand sequence. The spaces are guides for the eye ONLY. 3'- ATC AGT ATG ATG TGA CGT CAT AGC GTA TTG TCA G -5' 3'- ATC AGT ATG ATG TGA CGT CAT AGC GTA TTG TAC AG-5' Write the one-character polypeptide sequence for the protein. Include any start or stop codons. What kind of mutation has this insertion caused ("insertion" cannot be one of your answers)?

Explanation / Answer

DNA coding sequence: 3' - ATC AGT ATG ATG TGA CGT CAT AGC GTA TTG TCA G -5'

RNA strand:                   5' - UAG UCA UAC UAC ACU CGA GUA UCG CAU AAC AGU C - 3'

Single stranded polypeptide: STOP S Y Y T R V S H N S

Mutated sequence : 3' - ATC AGT ATG ATG TGA CGT CAT AGC GTA TTG TAC AG -5'

RNA strand:                   5' - UAG UCA UAC UAC ACU CGA GUA UCG CAU AAC AUG UC - 3'

Single stranded polypeptide: STOP S Y Y T R V S H N M(START)

The insertion of a base resulted in frame shift mutation resulting in altered DNA sequence and altered polypeptide.

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