In the following hydropathy plot of ACAT-1 (according to Kyte and Doolittle (49)
ID: 82831 • Letter: I
Question
In the following hydropathy plot of ACAT-1 (according to Kyte and Doolittle (49). Lin S et al. J. Biol. Chem. 1999;274:23276-23285 copyright 1999 by American Society for Biochemistry and Molecular Biology) what is the significance of the y-axis? Why do biochemists use hydropathay plots? Draw the structure that would be found in an aqueous solution at pH 7 of the following, give the 3 letter abbreviation and 1 letter code, and indicate whether the indicated amino acid is hydrophilic or hydrophobic: a) an aromatic amino acid; b) a sulfur containing amino acid; c) a cyclic saturated secondary amino acid; d) an acidic amino acid; e) a basic amino acid; f) an amide amino acid; g) a branched chain amino acid. In a mixture of the five proteins listed below, what is the order of migration in an SDS-polyacrylamide gel electrophoresis experiment? (M_r means relative molecular weight. Which should migrate the farthest, then 2^nd, etc.?) cytochrome c, M_r = 13,000 ribonuclease A, M_r = 13, 700 myoglobin, M_r = 16,000 histidine decarboxylase, M_r = 33,000 serum albumin, M_r = 68, 500Explanation / Answer
8)
The Y axis in hydropathy plot represents hydrophobicity or hydrophilicity depending on the R group present on the AA.
Hydropathy plots are used to display hydrophilic or hydrophobic regions of the protein sequence and on the that basis structure of the proteins could be predicted based on these regions.
It also tells about the type of amino acid when placed in organic solvent and or polar solvent( the more positive value on plot probably be membrane spanning alpha helix presenting an hydrophobic segment.
5)
In SDS-PAGE cytocrome move farthest ( at bottom )
then ribonuclease A
then myoglobin
then histidine decarboxylase
the serum albumin (at top of gel)
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