can someone please help me with the right answer for the following questions. ca
ID: 83772 • Letter: C
Question
can someone please help me with the right answer for the following questions. can you please answer all of them or dont attempt to answer. thank you.
Polyacrylamide gel electrophoresis (PAGE) makes use of a synthetic polymer which it is pr to control th pore size of the gel. Which ONE of the e New four parameter M has most uence on the pore size? Percentage of acrylamide Percentage of methylene-bis acrylamide. C. The concentration of ammonium persulfate. The concentration of TEMED (1 mark) Staining refers to the means by which protein bands on a gel can be visualised and quantified. Which ONE of the following is not used as a stain? Coomassie blue. Ponceau S Silver salts Bromophenol blue. (1 mark) Blotting refers to the method of quantitation devised by E. M. Southern, wh has been u to quantify DNA, RNA and proteins Whick ONE of the sed h following statements about blotting is correct? uth ern blotting is for proteins. Western blotting is for DNA. North ern blotting is for RNA. North ern blotting is for DNA. (1 mark) SDS-PAGE is a technique which enables the molecular weight of a protein to ONE of the following descriptions best describes how be determined. Wh separation occurs in SDS-PAGE? SDS binds to proteins producing an electrically neutral rod-shaped complex the size of wh ch is dependent upon the RMM of the protein Separation is therefore due to the sieving effect of the gel SDS binds to proteins producing an electrically neutral rod-shaped complex the size of wh ch is dependent upon the RMM of the protein. Separation is therefore due to electro-osmotic forces SDS binds to proteins producing an electrically neutral rod-shaped complex the size of wh ch is dependent upon the RMM of the protein Separation is therefore due to electrophoretic forces. D. SDS binds to proteins producing an electrically neutral rod-shaped complex the size of wh ch is dependent upon the RMM of the protein. Separation is therefore due to the absorption of the complex on the gel wh ch acts as a stationary phase.Explanation / Answer
5. Ans: A. Percentage of Acrylamide.
The role of bis acrylamide is to join the two acrylamide to prepare the mesh, but increase in numner of acrylamide molecules help to decrease the pore size.
6. Ans. D. Bromophenol blue.
All other examples use to stain protein gel of PVDF/ nitrocellulose membrane other than Bromophenol blue. It use as a gel loading dye in SDS PAGE.
7. Ans. C. Northern blotting for RNA.
Southern blotting for DNA, Western blotting for protein.
8. Ans. A. SDS binds to proteins producing an electrically neutral rod shaped complex the size of which is dependent upon the RMM of the protein. Separation therefore due to sieving effect of the gel.
One correction in last question, in all options it is mentioned that ‘SDS binds to proteins producing an electrically neutral rod shaped complex ‘ whereas due to binding of SDS to protein it does not produce neutral rod shaped complex but produce negatively charged rod shaped complex.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.