please answer as much as you can, thank you 6. You perform a western press-blot

Question

please answer as much as you can,
thank you

6. You perform a western press-blot to dewect albumin from rat serum You are convinced that you did every single step of the protocol perfectly Yet, when you did the color development reaction you find that arr the Which step in every turned purple Thus, did get a specific reaction for albumin a) You do you think you did wrong? you not You forgot to add the in your color devel sol did not lopment b press hard enough to allow transfer of proteins to the trocellulose membrane You forgot to d) All of the above 7. You have generated an antbody to a human protein called Nesprin 1 by injecting purified human Nesprn into a To Nesprin-1 from cells western blot press which HRP (horse radsh anti-rabbit secondary antibody would you use? b) rat anti-mouse c) rabbit-anti ral d) mouse anti-rabbit 8 Which of the following about ectins? a) Lectins are carbohydrate binding prateins b) Lectins play in signaling and cell-cel interactions c) Receptors for lectins are cell surface glycoproteins he above 9. During the immunohistochemistry lab yau performed which of the following solutions dissolved the embedding compound and allowed antibody penetration? a) Iso propanol b) histoclear (clearing solution) c) gelatin d) TBS buffer

Explanation / Answer

8) Lectins are carbohydrate binding proteins and they play role in cell signalling and cell inteactions but notg completely as like immune system.Coming to the receptors for lectin are cell surface glyco proteins.So i think all the given options are correct.

12) Yes it is TRUE because the main role of pholem in plants is,it carries food from leaves to the various part sof the plant.

13) False because the SDS is a kind of detergent which disrupts the territory structure of proteins.So they SDS has effect on protein structures.

15) Yes the nuclear staining is followed by counterstaining of alchoholic solution of esoin Y.So the given statement is true.

17) Yes SDS treated proteins have uniform negative charge because they impart two negative charges to every molecules in SDS.So the given statement is TRUE

Related Questions

Navigate

• Browse (All)
• Browse P
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.