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separation CE CY 35) You are given a yeast temparature-sensitive cell cycle muta

ID: 84435 • Letter: S

Question

separation CE CY 35) You are given a yeast temparature-sensitive cell cycle mutant strain. The haploid (1N) strain grows fine at the permissive temperature, but at the restrictive temperature, all of the yeast cells in the culture stop growing and accumulate as two large cells still joined together, with a 2N DNA content. What does this tell you about the gene that has been mutated? a) it encodes a protein that is required for DNA replication b) it encodes a protein that is required for mitosis c) it encodes a mutant protein that is inactive at both permissive and restrictive temperatures d) it encodes a protein that is required for cytokinesisi e) it encodes a protein required at the G1/S transition

Explanation / Answer

1) At the restrictive temperature, all the yeast cells in the culture stop growing at restrictive temperature and still joined together, therefore accumulate as two large cells with a 2N DNA content.

So answer is B

It encodes a protein that is required for mitosis

2) answer is D

The couple, M2/F2, who claim they are innocent of the abduction as child one band is similar to mother and one to father