Answer the following questions with respect to the tryptophan operon in Escheric

Question

Answer the following questions with respect to the tryptophan operon in Escherichia coli. a) Is the trp operon regulated by induction or repression? Explain your answer. b) Is TrpR a positively- or negatively-acting protein? Explain your answer. c) Explain the term "coupled transcription and translation" d) Explain attenuation, using a diagram to help your explanation; You must include diagrams (visual representation is an important skill) Your diagrams need to show what is going on at; i. Lower concentrations of tryptophan, and iii. Higher concentrations of tryptophan You must include sufficient text to explain your diagram such that it helps a fellow student understand attenuation. In addition: -Include the ribosome, and state what its role is in attenuation. -Include the RNA polymerase, and state what its role is in attenuation.

Explanation / Answer

a) The trp operon is a repressor operon, its regulation is depending on the level of tryptophan. When the tryptophan level is high, a reppressor protein get bind at the operator sequence with the help of corepressor and repress the trp operon there by inhibiting RNA polymerase enzyme from transcribing trp related genes.

c) Coupled transcription and translation is the combined process of transcription and translation together at the same time. It is the specific intimate process which make prokayotes different from eukaryotes. Here the translation begins while the mRNA still being synthesized. Which means that mRNA synthesis and Protein synthesis occurs at the same time.

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