You have three pure cultures of bacteria. One is Gram positive bacteria and the

Question

You have three pure cultures of bacteria. One is Gram positive bacteria and the other two are Gram negative bacteria but you have mixed up the labels. You know that one of the Enteric bacteria can ferment glucose and produce ethanol and CO_2. The other can ferment glucose and produce lactic acid. Describe the general procedure you could perform to determine of the three cultures which one is which? A one gram sample of hamburger is diluted to 10^-4 in sterile saline. One milliter of the 10^-6 dilution is spread on a nutrient agar plate and incubate. In two days, the plate is examined and 54 colonies are counted. How many bacteria are present in the meat? ___ A student prepares a gram stain of an unknown culture and observes that it is gram negative. He asserts with great certainty. "It's Escherichia coil" Another student exclaims, "No, it's Kiebsiella pneumoniae." As a student who has taken a microbiology course, what would you tell them about the validity of the statements?

Explanation / Answer

Question 7: The answer is
54 * 10 * 10 * 10 * 10 * 10 * 10 = 54000000 cfu/ gram humburger.

ACCRODING TO CHEGG GUIDELINES WE HAVE TO ANSWER ONE QEUSTION AT A TIME. POST THE REST AS SEPERATE QEUSTIONS THEN I CAN HELP YOU.

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