Add 4 mL of H2O and 2 mL ethyl acetate to a screw capped centrifuge. Add 4 drops

Question

Add 4 mL of H2O and 2 mL ethyl acetate to a screw capped centrifuge.

Add 4 drops of solution A (blue) to the centrifuge tube. Shake for 30 seconds. Describe the color of each layer

3. Add 2 drops of 1M HCI. Let solution sit for 1 min. Then shake for 1 min, using a rapid rock motion. Describe the color of each layer

4. Add 4 drops of 1 M NaOH, shake again for 1 min. Describe the color of each layer.

Step#2

Ethyl Acetate

Aqueous

red

clear

Step#3

Ethyl Acetate

Aqueous

red

clear

Step #4

Ethyl Acetate

Aqueous

blue

clear

Describe fully what occurred in steps 2,3, and 4.

The nature (cation, anion, neutral species) of the inorganic compound.

Step#2

Ethyl Acetate

Aqueous

red

clear

Step#3

Ethyl Acetate

Aqueous

red

clear

Step #4

Ethyl Acetate

Aqueous

blue

clear

Explanation / Answer

Thin-layer chromatography (TLC) is a chromatography technique used to separate non-volatile mixtures.[1] Thin-layer chromatography is performed on a sheet of glass, plastic, or aluminium foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide, or cellulose. This layer of adsorbent is known as the stationary phase.

After the sample has been applied on the plate, a solvent or solvent mixture (known as the mobile phase) is drawn up the plate viacapillary action. Because different analytes ascend the TLC plate at different rates, separation is achieved.[2]

Thin-layer chromatography can be used to monitor the progress of a reaction, identify compounds present in a given mixture, and determine the purity of a substance. Specific examples of these applications include: analyzing ceramides and fatty acids, detection ofpesticides or insecticides in food and water, analyzing the dye composition of fibers in forensics, assaying the radiochemical purity ofradiopharmaceuticals, or identification of medicinal plants and their constituents [3]

A number of enhancements can be made to the original method to automate the different steps, to increase the resolution achieved with TLC and to allow more accurate quantitative analysis. This method is referred to as HPTLC, or "high-performance TLC".

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.