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Enhancers: a) are always located upstream from the TATA sequence B) function in

ID: 84965 • Letter: E

Question

Enhancers: a) are always located upstream from the TATA sequence B) function in only one orientation C) can be 2000 bp away from the TATA sequence D) all of the above In eukaryotes, transcription factors: A) bind to the promoter before RNA polymerase binds B) bind directly to RNA polymerase NOT to the DMA C) bind to the operator NOT to the promoter D) are NOT required for transcription In eukaryotes, formation of mature mRNA ______ RNA editing, that is, changing bases in the exon sequences. a) sometimes involves b) never involves Human gene therapy in the treatment of severe combined immunodeficiency (SCID) did NOT involve: a) use of a modified retrovirus as vector b) injecting the virus carrying the ADA gene directly into the patient c) use of tissue culture Histone acetyl transferase enzymes(HATs): A) acetylate histone proteins to decrease transcription B) acetylate histone proteins to increase transcription C) deacetylate histone proteins to increase transcription D) none of the above In the lactose operon, the "z" gene codes for the enzyme beta-galactosidase, "o" is the operator gene and "i" is the repressor gene. If an E. coli strain with the genotype i'o'z'/F' is grown on medium which contains no lactose or glucose, beta-galactosidase _______ produced. a) is b) is not cDNA's _______ introns. a) contain b) do not contain

Explanation / Answer

26. Enhancers can be 2000 bp away from TATA box.

EXplaintion. Enhancers are transcription control elements may be more than 10 kilobases away from the transcription start.It activates transcription independent of its orientation.These may function either upstream or downstream of the initiation site.

27. Binds to promoter before RNA polymerase binds.These bind to the promoter region and then help in mediating recruitment of RNA polymerase.

28 Sometimes involves.

In eukaryotes RNA editing is widespread, occuring in organisms as diverse as yeast and humans.

29.use of tissue culture.

30.Acetylate histone proteins to increase transcription.Acetylation decreases contact of DNA with the nucleosomes and makes it more accessible to transcription factors to bind and hence promotes transcription.

31.The question is not so clear but i think there is positive sign on all the genes.so if the repressor is present and there is no lactose or glucose in the medium, repressor will not be inactivated and will bind freely to the operator and will thus prevent RNA polymerase from binding to the promoter and hence no expression of beta galactosidase will occur.

32.cDNA does not contain introns.