The plot of 1/Vo versus 1/[S] is sometimes called a Lineweaver-Burk plot. Anothe

Question

The plot of 1/Vo versus 1/[S] is sometimes called a Lineweaver-Burk plot. Another way of expressing the kinetic data is to plot Vo versus Vo/[S], which is known as the Eadie-Hofstee plot.

a) Rearrange the Michaelis-Menton equation to give Vo as a function of Vo/[S]

b) What is the significance of the slope, the vertical intercept, and the horizontal intercept in a plot of Vo versus Vo/[S]

c) Sketch a plot of Vo versus Vo/[S] in the absence of an inhibitor, in the presence of a competitive inhibitor, and in the presence of a noncompetitive inhibitor

Explanation / Answer

(A) 31.1

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