5. The hydration of CO2 catalyzed by the enzyme carbonic anhydrase is given by t

Question

5. The hydration of CO2 catalyzed by the enzyme carbonic anhydrase is given by the following reaction: CO2 + H2O arrow HCO3^- + H^+ In this reaction, CO2 is converted to bicarbonate ion. Bicarbonate is transported in the bloodstream and converted back to CO2 in the lungs, a reaction that is also catalyzed by carbonic anhydrase. The following initial reaction rates for the hydration reaction were obtained for an initial enzyme concentration of 2.3 nM and temperature 0.5 degree C Determine Km and kb for the enzyme at this temperature.

Explanation / Answer

Plot a graph between conc of CO2 on x axis and rate on y axis. You will get a staright line with c (intercept on Y) as rmaximum rate which is 2.5E-4 M/s. Given conc of enzyme is 2.3nM

so using Michaelis

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