Adipose-derived stem cells were transduced with a retroviral vector containing t

Question

Adipose-derived stem cells were transduced with a retroviral vector containing the full coding sequence for hepatocyte growth factor. The cells were cultured on two dimensional tissue culture polystyrene and on porous three-dimensional collagen sponges, and maintained in chemically-defined medium previously shown to induce hepatogenic differentiation. The gene expression profile of cells seeded on the porous collagen sponges indicated significantly higher levels of liver-specific gene i.e, albumin, cytochrome P450) compared to those cultured on two-dimensional tissue culture polystyrene. (a) Aside from differences in substrate mechanical properties, describe two intrinsic material characteristics that differ between the tissue culture polystyrene and the collagen sponges that may be responsible for the elevated expression of hepatocyte differentiation markers by cells seeded on the collagen sponges. (b) Design an experiment to determine which of the two material characteristics is most likely responsible for the observed increase in hepatocytic differentiation by cells seeded on the collagen sponges.

Explanation / Answer

b Trypsin treatment of the collagen treated cells will lead to detachment of these cells. If after trypsin treatment the differentiation of the hepatocytes is affected we can prove that the observed differences in differences in differentiation in polystyrene and the collagen coated tissue culture plates is due to attachment

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