Show your work. a) Describe how to make 10mI of a 1:50 dilution from a 1 times 1

Question

Show your work. a) Describe how to make 10mI of a 1:50 dilution from a 1 times 10^12 cells/ml stock. b) What is the resulting cell concentration? How much of a 20 mg/ml stock solution of ampicillin would you need to add to 500 ml of bacterial growth medium to achieve a final concentration of 100 mu g/ml ampicillin? Show your work. You have a stock solution of 20X TAE Buffer. "20X" refers to the solute concentration of the stock relative to the final "working concentration". How would you prepare 1L of IX TAE Buffer from the 20X TAE stock? Show your work.

Explanation / Answer

7.C1V1 = C2V2 or Concentration-1 X Volume-1 = Concentration-2 X Volume-2

1000 x V1= 2 X10ml

v1=0.02 in 10ml

b. cell concentration will be diluted by 10 times

8. C1V1 = C2V2 or Concentration-1 X Volume-1 = Concentration-2 X Volume-2

(20 mg/ml) V1 = (0.1 mg/ml)(500 mL)
20 V1 = 50

V1 = 50/20 = 2.5mL

9. C1V1 = C2V2 or Concentration-1 X Volume-1 = Concentration-2 X Volume-2

(50X) V1 = (1X)(1000mL)
(20X) V1 = 1000

V1 = 1000/20 = 50mL

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