14. DNA fragments from a gel transferred to a paper during the procedure called

Question

14. DNA fragments from a gel transferred to a paper during the procedure called Southern blotting. The purpose of trans the DNA from a gel to a nitrocellulose paper is to: Permanently attach fragments to a substrate B) Separate the two complementary DNA strands. C Transfer only the DNA that is of interest. D) Prepare the DNA for digestion with restriction enzymes. E)Separate out the PCRs. 15. The Energy needed during translation is mostly a) ATP b) CTP c) GTP d) TTP 16. Which of one of these is not a step while conducting the process of PCR? A) Denaturation B) Annealing Elongation D) All the options Av B, C and E are involved in the process of PCR E) mRNA is transferred to nitrocellulose membrane. 17. The nucleotides produced by the action of restriction enzymes on plasmid DNA are specifically referred to as A) Sticky ends. B) Blunt end C) Triple Stranded DNA D) Restriction fragments. E) Ligases 18. The energy used by enzyme tRNA Synthetase is a) ATP b) GTP c) No energy is required d) All of the above 19. The polymerase chain reaction (PCR) has been used to amplify DNA from which of the following? A) fossils B) fetal cells C) viruses D) bacteria E) all of the above 20. Dideoxy ribonucleotide chain-termination is a method of A) cloning of DNA. B) Sequencing of DNA. C) Digestion of DNA. D) Synthesis of DNA.

Explanation / Answer

14. A. Permanently attach the DNA fragments to a substrate.

15. C.GTP

16. E. mRNA is transferred to nitrocellulose membrane

17. A. Sticky ends

18. D. All of the above

19. E.all of the above

20. B. Sequencing of RNA

Sanger's method.

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