because italowesus 6 The polymerase chain reaction is A) insert eukaryotic genes
ID: 86979 • Letter: B
Question
because italowesus 6 The polymerase chain reaction is A) insert eukaryotic genes into prokaryotic plasmids B) Incorporate genes into viruses Make DNA from RNA transcripts. D) Make many copies of a targeted segment of DNA E)Insert regulatory sequences into eukaryotic genes. once, more than once, or not at all. restriction enzymes B. gene cloning DNA ligase D. gel electrophoresis reverse transcriptase E 7. Produces multiple identical copies ofagene for basic research or for large-scale production of a gene 8. separates molecules by movement due to size and electrical charge 9. Seals the sticky ends of restriction fragments to make recombinant DNA Answer: 10. is used to make complementary DNA (cDNA) from RNA 11. cuts DNA molecules at specific location or sequence Answer: 12. The fragments of DNA are typically separated from one another by which process? A) Filtering B) Centrifugation C) Gel electrophoresis D) PCR E) Electron microscopy 13. The Codon: anticodon recognition takes place on a) Small subunit of ribosomes b) Large subunit of ribosomes c) Both a) and b) d) None of the aboveExplanation / Answer
6 option D makes multiple copies of a targeted segment of DNA
PCR is meant for amplifying DNA of a particular length depending on the primer of choice
7. gene Cloning
8. Gel electrophoresis
9 DNA ligase
10. reverse transcriptase
11. restriction enzyme
12. PCR here the DNA strands are separated by heat and the restriction enzyme is added to aid amplification of the desired DNA fragment.
Gel electrophoresis will force the DNA molecule to move based on charge and will not separate the Strands. Similarly centrifuge if high speed will shatter the DNA into pieces and not strand separation
13. option B large unit of Ribosome. The mRNA is bound to ribosome only in the large unit and the codon anticodon recognition happens here when the tRNA binds to the mRNA
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