and Prokaryote DNA polymeraees 4-7 Catalytic properties vitro were studied in Fi
ID: 87302 • Letter: A
Question
and Prokaryote DNA polymeraees 4-7 Catalytic properties vitro were studied in Figure A in DNA duplex shown in questions) ubunt added Doubator Each sample was divided into two aliquots, and he four triphosphates (dATP, dTTP dGTP and added and further incubated for 90 seconds presence Pola) of denaturing (oonditions that cause DNA autoradiography. The size of the bands are: a, 22 gel followed by nucleotides, b. 16 nucleosides and c, 15 Choose from the following five options to answer questions 4-7. An option may be used more than once. Molecules in band a in band b Molecules in band c band a, b, and c E. Molecules in none of the bands, neither a b, nor c 4. They are double-stranded DNA molecules. 16 nucleotides 3' GGA, TG TT TC 30 nucleotides 24 81216 0 2 4 81216 c subunit (min) 01 Pola. DNA polymerase Pola 1 2 3 4 5 6 7 891011121314 Sample A Molecules in band a B Molecules in band b C Molecules in band c D Molecules in band a, b, and c E Molecules in none of the bands, neither a, b, nor c.Explanation / Answer
4. band c since circular DNA withour any nick will move faster.
5. Answer is neither; Since DNA polymerase involve in the synthesis and no any new band appear in presence of DNA polymerase
6. band a, exonuclease cleave nucleotide from terminal end and we can view new band formation in lane 1 band a that depict nucleotide single strand generated after cleavage from terminal end of DNA and resulting in position in lane 1and a
7. A & D 9 Adenine and Uracil. 3' end is a region of the DNA which is transcribed into mRNA, sequences which are required for the addition of the poly(A) tail to the message, including the hexanucleotide AAUAAA.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.