Suppose we have two proteins in the same solution and we want to separate them f

Question

Suppose we have two proteins in the same solution and we want to separate them from each other. I'll give you some properties of the two enzymes and then ask a question about how to best separate them.

Protein A: 160 amino acid residues; 5 Asp, 2 Glu, 2 Lys, 5 Arg and 6 Tyr are among these residues.

Protein B: 155 amino acid residues; 2 Asp, 2 Glu, 2 Lys, 5 Arg and 9 Tyr are among these residues.

Also, let's say that we don't know anything about "ligands" that bind to either of these proteins.

Now, which method would work best to separate these from each other, working at pH 7?

A. gel permeation chromatography

B. anion exchange chromatography

C. cation exchange chromatography

D. affinity chromatography

Explanation / Answer

C. Cation exchange chromatography

would be the best method to seperate the proteins at pH 7

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