Pre-Lab Questions 1. Read over the Appendix entitled Operation of a Spectrophoto

Question

Pre-Lab Questions 1. Read over the Appendix entitled Operation of a Spectrophotometer. Use the information provided there to answer the following questions. a. What is the difference between absorbance (A) and transmittance (T)? b. What is the proper way to handle a cuvette for use in a spectrophotometer? Why are these steps important for analysis? 2. When two solutions are combined, the concentration of each compound s diluted due to the addition of the other solution. To determine the diluted molarity of a compound, the following equation can be used: M1V1 = M2V2 M1 represents the original molarity of a solution, V1 represents the volume of solution before the dilution, M2 is the diluted molarity, and V2 is to total volume of the new dilute solution (not the volume of additional solution added). Using this equation, calculate the diluted molarity of loo mL of a 0.5 M solution when 50 mL of water has been added.

Explanation / Answer

2. 0.5*0.1=0.05*V

V=0.5*0.1/0.05=1 litre

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