A sample of blood (0.5 ml) was diluted with water to 100ml. The original blood h

Question

A sample of blood (0.5 ml) was diluted with water to 100ml. The original blood had a protein concentration of 5 mg/ml. One ml of the diluted blood was analyzed for catalase activity by measuring the colume of oxygen produced under standard conditions. One ml of the diluted blood yielded 5 ml of oxygen in 10 minutes. If an enzyme unit is defined as that amount of enzyme releasing 1 ml of oxygen in 1 min, calculate (a) the number of Units per ml of undiluted blood, and (b) the specific activity of catalase in the UNDILUTED blood.

Explanation / Answer

One ml of the diluted blood yielded 5 ml of oxygen in 10 minutes then 100ml of the diluted blood yielded 5 ml of oxygen in 10 minutes = 100*5 = 500 ml of oxygen in 10 minutes = the number of Units per ml of undiluted blood,

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