You have a single cell of one of the organisms below. Assume that the cell is in
ID: 88420 • Letter: Y
Question
You have a single cell of one of the organisms below. Assume that the cell is in a closed environment with only 3 molecules of glucose available to it and is currently in the lag state of the exponential growth curve, so binary fission is not possible. Also, let's simplify the situation further and ignore anabolic process that would consume ATP - they would be occurring, but it makes our calculations too complicated for the purpose of this exercise. Assume that O_2 is present, and if your organism uses it, that it is not at toxic levels. (Reminder:1 NADH produces 3 ATP, 1 FADH_2 produces 2 ATP) Show your work and calculate the energy generated (ATP) in: a ATP created by substrate level phosphorylation (SLP) from glucose? b. ATP created by oxidative level phosphorylation (OLP) from glucose? c. ATP overall from glucose? d. What happens if O_2 becomes unavailable - is ATP still made? If it is, how does this impact the overall ATP generation? Organisms: 1. Obligate aerobeExplanation / Answer
The overall ATP production with single glucose molecule under aerobic condition is given below,
Glycolysis:
2 ATPs are consumed during preparatory phase
Substrate level phosphorylation ------> 4
Oxidative phosphorylation -----------> 2 NADH (6 ATP)
Conversion of pyruvate to acetyl CoA:
Oxidative phosphorylation ------------> 2 NADH (6 ATP)
Kreb's cycle:
Substrate level phosphorylation -----------> 2
Oxidative phosphorylation --------------> 6 NADH (18 ATP) and 2 FADH (4 ATP)
Substrate-level phosphorylation is a metabolic reaction that results in the formation of ATP by the direct transfer of a phosphoryl group to ADP from another phosphorylated compound. Oxidative phosphorylation couples oxidation with phosphorylation. Here, the cells use enzymes to oxidise nutrients into energy.
a) The number of ATP produced by substrate level phosphorylation is 6
b) The number of ATP produced by oxidative phosphorylation is 34
c) The overall net ATP produced from one glucose molecule is 38 since 2 ATP molecules are consumed during preparatory phase of glycolysis
d) If oxygen becomes unavailable, only 2 molecules of ATP are produced by substrate level phosphorylation in glycolysis.
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