We are purifying RNA polymerase subunits from E coli and we believe our extract

Question

We are purifying RNA polymerase subunits from E coli and we believe our extract contains both the holoenzyme species with sigma70, and also the sigma70 subunit in free form. Which strategy is best suited to separate the holoenzyme species from the free sigma70 species? A1. Affinity chromatography, using promoter DNA attached to the resin. A2. CM - cellulose chromatography. A3. DEAE - cellulose chromatography. A4. Gel filtration chromatography. A5. None of the above would separate these two species. Which of the following statements is TRUE regarding specific activity during purification? AI. If specific activity remains the same for each purification step, this indicates the enzyme is not being destroyed by the purification procedure. A2. Specific activity has a value of 100% for a completely pure enzyme. A3. In typical purifications, specific activity will almost always decrease if you increase the number of purification steps. A4. "Specific activity" differs from "activity" because specific activity refers only to that specific enzyme being discussed. A5. All of the above are false.

Explanation / Answer

Which of the following statement is true regarding specific activity during purification?

all of the above are FALSE-------------answer

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Specific activity

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