protein assay requires the reaction of the protein solution with a protein-bindi

Question

protein assay requires the reaction of the protein solution with a protein-binding dye for one minute, followed by measuring the absorbance of the protein-dye complex at 505 nm. The absorbance data for four standard protein solutions and the blank is provided in the table below. The absorbance of each solution was measured three times. Construct a calibration curve using the data in the table, and find the slope (m), intercept (b), and their respective standard deviations using the method of least squares. Note that the calibration curve will be created from absorbance values corrected for the absorbance of the blank, and not the raw data in the table. Obtain the corrected data by subtracting the average absorbance of the blank values from each absorbance value in the table (including the individual blank values). When creating the calibration curve, do not use the average absorbance at each protein concentration. Instead, use each data point separately, meaning there will be three data points at each protein concentration, for a total of 15 points (including each individual blank value). Calculate the concentration and uncertainty of a protein solution that produced an average absorption of 0.345 when measured three times.

Explanation / Answer

y = mx + c

y = absorbance

x= concentration

c = intercept

m= slope = 0.0944

Standard deviation = 0.00153

intercept = -0.011

standard deviation = 0

given:

Absroption = 0.657

putting values

y = mx + c

0.657 = 0.944 X x -0.011

x = 0.707

Replicate1 Replicate 2 Replicate 3 average corrected1 Corrected 2 Corrected 3 Blank 0 0.0332 0.0331 0.0335 0.0333 -6.7E-05 -0.0002 0.0002 0.1 0.0935 0.0932 0.0931 0.0333 0.0602 0.0599 0.0598 0.2 0.22 0.22 0.22 0.0333 0.1867 0.1867 0.1867 0.5 0.496 0.5 0.499 0.0333 0.4627 0.4667 0.4657 1 0.965 0.966 0.964 0.0333 0.9317 0.9327 0.9307

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