Using the data provided, what is the Michaelis constant for glucose on this enzy

Question

Using the data provided, what is the Michaelis constant for glucose on this enzyme?

Expt. 1
Given 1.0 mM glucose, V0 = 0.250/sec

Expt. 2
Given .25 mM glucose, V0 = 0.135/sec

Expt. 3
Given .286 mM glucose, V0 = 0.151/sec

Expt. 4
Given .400 mM glucose, V0 = 0.180/sec

Expt. 5
Given .332 mM glucose, V0 = 0.165/sec

Expt. 6
Given .500 mM glucose, V0 = 0.188/sec

Expt. 7
Given 0.667 mM glucose, V0 = 0.212/sec

What is the maximum velocity for this enzyme?

A.) 0.40 mM
B.) 0.33 mM
C.) 0.25 mM
D.) 0.50 mM
E.) -3.0 mM
F.) 1.0 mM

Explanation / Answer

B) 0.33 mM

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