I need help with Biochem Hw pleas > try to solve step by step. thank you The kin

Question

I need help with Biochem Hw pleas > try to solve step by step.

thank you

The kinetics of on enzyme ore measured at different substrate concentrations (column A) in the absence of inhibitor (column C) and presence of inhibitor (E). On graph paper or using graphing software, plot velocity as a function of substrate concentration for the inhibited reaction. What is the approximate V_max? From V_max/2, determine K_m On the same graph, plot velocity as a function of substrate concentration in the presence of inhibitor. What is the approximate V_max? From V_max/2, determine K_m. On the same graph, plot velocity as a function of substrate concentration in the presence of inhibitor. What is the approximate V_max? From V_max/2, determine K_m. From this plot, does the inhibitor appear to be competitive or noncompetitive? Fill in columns B, D and F in the table by calculating the reciprocals of the values in columns A, C, and E, respectively. On a new graph, plot 1/velocity as a function of 1/[substrate]. This is a called a plot. From this plot, estimate 1/V_max and 1/K_m in the absence and presence of inhibitor From this plot, does the inhibitor appear to be competitive or noncompetitive?

Explanation / Answer

6a)

Vmax = 40.5 Km = 0.1 nearly

b)

Vmax = 33.8 Km = 0.03 approx

c) we can determine this from graph of 1/V and 1/S

d)

0.03

e) Lineweaver-Burk plot

1/ Vmax = intercept =0.0224

x -intercept = -1/Km

S 1/S v 1/V Vinh 1/Vinh 3 0.33 10.4 0.10 4.3 0.23 5 0.20 14.5 0.07 6.4 0.16 10 0.10 22.5 0.04 11.3 0.09 30 0.03 33.8 0.03 22.6 0.04 90 0.01 40.5 0.02 33.8

0.03

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