pipetting pleaseeee help me Was it more accurate to measure 200 mu L re to measu
ID: 893409 • Letter: P
Question
pipetting pleaseeee help me Was it more accurate to measure 200 mu L re to measure 1 ml with the P1000? Explain your answer, indicating what you compared or analyzed to answer this question. Was it more precise to measure 1 ml with the P1000 or the P5000? Explain Was it more accurate to measure 200 mu L with the P200 or with the P1000? Explain. Was it more precise to measure 200 mu L with the P200 re with the P1000? Explain. If you had to measure 100 mu L, would you he more likely to use the P200 or the P1000? I Explain. What mass would 2 mu L of water have? Can you guess why you did not check the precision re accuracy of your P2 Pipetmen? How do you make 200 mL of a 0.150 M solution of Tris-base ( MW 121 I g/mol)? How do you make 50 mL of a 6% (wt/vol) casein solution? How do you make 250 mL of a 25%. (vol/vol) glycerol solution? How do you make 400 mL of a 33 mM aspartate (MW = 133.11 g/mol) solution? Practice with serial dilutions Solution A = Take 5 mL of 0.25 MTris-base solution and add 45 ml of water. What is the concentration Solution A? Take 5 mL of Solution A aid dilute it to a total of 50 mL. What will be the molar (M) concentration of the new solution? Express the concentration of the final solution in both millimolar (mM) concentration and micromolar (mu M) concentration.Explanation / Answer
Following table will give you idea of ranges of pipeete volume :
Now, A small volume range of a pipette like 10 - 100 µl results in a much higher accuracy than a broad range from 0.1 - 1,000 µl per pipette
Using this concept
2.P1000 is more accurate for 200 µl.
3.P5000 is more precise to measure 1ml as compare to P1000
4.P200 is more accurate for 200 µlthan P1000.
5.P1000 is more precise for 200 µl than P200.
6.If accuracy is concerned P200 if precision is concerned P1000
7.Because no pipet other than P2 can have such precision and accuracy for 2 µl volume.
8. mass of tris base=121.1*0.2*0.15=3.633 gm
3.633 gm of tris base is taken in 1 litre volume
9.mass of casein =50/1000*0.06=3mg
so 3mg of casein is taken in 1 litre
10.volume of glyceral=250*0.25=62.5ml
so 62.5ml is taken in 1 litre solution
11.mass of aspartate=33*400*10^-6*133.11=1.757052gm
so 1.757052 gm of aspartate in 1 litre volume
12.
a.concentration of solution a=5*0.25/50=0.025
b.concentration of new solution=5/50 or 0.1 times concentration of old solution=0.1*0.25=0.025
c.25mM,25000µM
Name Min. volume (µL) Max. volume (µL) Colour on Gilson tip size (µL) P2 0.2 2 Orange 10 P10 1 10 Red 10 P20 2 20 Lemon 200 P100 20 100 Salmon 200 P200 50 200 Yellow 200 P1000 200 1000 Blue 1000 P5000 1000 5000 Purple 5000 P10000 1000 10000 Sky 10000Related Questions
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