What is the type of inhibition created by Inhibitor 1 ( I-1)versus Inhibitor 2 (
ID: 893850 • Letter: W
Question
What is the type of inhibition created by Inhibitor 1 ( I-1)versus Inhibitor 2 ( I-2)outlined for the data shown in the Table below? Provide data plots based on your analyzing of these data by a linear transformation of these data that substantiates your interpretation
[ substrate], micromolar
V, when no inhibitor, micromoles/min
V in the presence of I-1
Micromoles/min
V in the presence of I-2
Micromoles/min
3
10.4
4.1
2.1
5
14.5
6.4
2.9
10
22.5
11.3
4.5
30
33.8
22.6
6.8
90
40.5
33.8
8.1
What is the mechanism of inhibition of the enzyme whose kinetic properties are shown in the Figure when :
a. Inhibitor I1 is present? Why do you say this??
b. Inhibitor I2 is present? Why do you say this??
c. Inhibitor I3 is present? Why do you say this??
[ substrate], micromolar
V, when no inhibitor, micromoles/min
V in the presence of I-1
Micromoles/min
V in the presence of I-2
Micromoles/min
3
10.4
4.1
2.1
5
14.5
6.4
2.9
10
22.5
11.3
4.5
30
33.8
22.6
6.8
90
40.5
33.8
8.1
Explanation / Answer
Figure is not given
I have drawn the graph
So the Km values without inhibitor and with inhibitor I2 are same it will be Non competitive
Substrate 1/S V (no inhbitor 1/V V (I1) 1/V V (I2) 1/V 3 0.33 10.40 0.10 4.1 0.24 2.10 0.48 5 0.20 14.50 0.07 6.4 0.16 2.90 0.34 10 0.10 22.50 0.04 11.3 0.09 4.50 0.22 30 0.03 33.80 0.03 22.6 0.04 6.80 0.15 90 0.01 40.50 0.02 33.8 0.03 8.10 0.12Related Questions
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