A protein has a molecular mass of 200 kDa as determined by gel filtration (=nati

Question

A protein has a molecular mass of 200 kDa as determined by gel filtration (=native molecular weight). When separated by SDS-PAGE (without a reducing agent present) only one polypeptide appeared with a mass corresponding to 100 kDa. When determined by SDS-PAGE in combination with a reducing agent (DTT) exactly two polypeptides appeared of sizes 40 and 60 kDa. What is the correct structure of the native protein? Correct ans: The native protein is made of two 40 kDA and two 60 kDA polypeptides

Would like an explanation as to how this is correct and how similar questions would be solved

Explanation / Answer

Answer:

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.