Calculate the required volume of 0.25mg/mL BSA standard to reach to each concent

Question

Calculate the required volume of 0.25mg/mL BSA standard to reach to each concentration and fill the blanks in the table. Total volume in each tube is 250 microliters and each tube should have the same amount of PBS buffer as 5 microliters.

Table: Components for each protein standard solution of BCA assay

Please keep correct units.

Tube Number Concentration of BSA Standard (g/mL) Volume of 0.25mg/mL BSA standard (L) Volume DDI water (L) Volume of PBS Buffer (L) 1 Blank 0 245 5 2 10 5 3 20 5 4 30 5 5 40 5 6 50 5 7 80 5 8 100 5 9 150 5 10 200 5

Explanation / Answer

We wil use formula,

C1V1 = C2V2

lets say we need a 250 microliter of 10 microliter/ml BSA concentration

original solution has 25mg/ml = 25000 microgram/ml concentration

25000 x V1 = 10 x 250

V1 = 0.1 microliter

Similarly we fill the other columns

Tube No.    conc.. of BSA std.(microg/ml)     Vol. of BSA std (microL)

    1                                 0                                           0

    2                                10                                         0.1

    3                                20                                         0.2

    4                                30                                         0.3

    5                                40                                         0.4

    6                                50                                         0.5

    7                                80                                         0.8

    8                               100                                        1.0

    9                               150                                        1.5

   10                               200                                        2.0

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