It is suggested that an important region of an enzyme is highly dynamic and does
ID: 90014 • Letter: I
Question
It is suggested that an important region of an enzyme is highly dynamic and does not adopt any significant secondary structure. Which biophysical method would you use to support this hypothesis and why?
X-ray crystallography would be most appropriate as it is the only method that can determine an atomic resolution image of a protein molecule.
Circular dichroism spectroscopy would be most appropriate as it can give information on the primary structure of the protein.
Nuclear magnetic resonance (NMR) spectroscopy would be most appropriate as it generates an ensemble of all possible structures that exist in solution.
Absorbance spectroscopy would be most appropriate as it can allow the concentration of the protein molecules in solution to be determined.
Gel filtration chromatography would be most appropriate as it can distinguish between proteins based on their molecular weight.
a.X-ray crystallography would be most appropriate as it is the only method that can determine an atomic resolution image of a protein molecule.
b.Circular dichroism spectroscopy would be most appropriate as it can give information on the primary structure of the protein.
c.Nuclear magnetic resonance (NMR) spectroscopy would be most appropriate as it generates an ensemble of all possible structures that exist in solution.
d.Absorbance spectroscopy would be most appropriate as it can allow the concentration of the protein molecules in solution to be determined.
e.Gel filtration chromatography would be most appropriate as it can distinguish between proteins based on their molecular weight.
Explanation / Answer
Answer: Option C. Nuclear magnetic resonance (NMR)
The chemical shift obtained from NMR can be used to determine the 3-D structure of the protein, including the secondary structure, which would give us the necessary information regarding the active asite.
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