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pling You wish to measure the concentration of methyl benzoate in a plant stream

ID: 900289 • Letter: P

Question

pling You wish to measure the concentration of methyl benzoate in a plant stream by gas chromatography. You prepare a sample of butyl benzoate to use as an internal standard. A preliminary run, shown at the right involved a solution containing 1.27 mg/mL of methyl benzoate (peak A) and 1.40 mg/mL of butyl benzoate (peak B). The area of peak A was determined to be 307 and of peak B to be 353 (measured in arbitrary units by the computer). To measure the sample, 1.00 mL of a standard sample of butyl benzoate containing 2.75 mg/mL was mixed with 1.00 mL of the plant stream material, and analysis of the mixture gave a peak area of 479 for peak A and 401 for peak B. What is the concentration of the methyl benzoate in the plant stream? Number mg/mL conc. methyl benzoate 10 Time (min) Map deb

Explanation / Answer

As we can see that the peak area is continously increasing in a sample if it contains more of the methyl benzoate !

So in this case as we see that the peak area has increase to 479 and 401 so ,

The concentration of methyl Benzoate will be surely greater than (1.27+1.40)= 2.67mg/ml