Hello could you help me with the following questions. 1. Which strategy for test

Question

Hello could you help me with the following questions.

1. Which strategy for testing gene function requires that you know more about the gene or pathway you are studying before you start?

a. Forward genetics

b. Reverse genetics

2. Can the Cre-loxP system be used to "knock out" a gene in a wildtype (not genetically modified) mouse?

a. Yes, the Cre recombinase can use any loxP sites, even those that have not been engineered into the genome.

b. No, the Cre-loxP system requires loxP sites to be inserted into the genome by researchers.

3. What is the mechanistic reason that in situ hybridization images are purple?

a. Binding of RNA to a probe results in a purple precipitate.

b. An antibody conjugated to alkaline phosphatase cleaves NBT/BCIP into a purple product.

c. NBT/BCIP in solution is purple.

d. Digoxigenin-labeled nucleotides in the probe react with NBT/BCIP to form a purple product.

4. In situ hybridization detects:

a. RNA

b. Both RNA and protein

c. protein

d. DNA

5. Which is NOT a mechanism for control of mRNA expression by cytoplasmic localization?

a. Localized protection

b. Nuclear export and splicing

c. Active transport along the cytoskeleton

d. Diffusion and local trapping or anchoring

Explanation / Answer

1. (a) Forward genetics is useful for indentifying a set of genes responsible for a particular phenotype of an organism whereas, reverse genetics is useful for analyzing the phenotype of an organism after the disruption of a known gene.

2. Can the Cre-loxP system be used to "knock out" a gene in a wildtype (not genetically modified) mouse?
b. No, the Cre-loxP system requires loxP sites to be inserted into the genome by researchers.

3. What is the mechanistic reason that in situ hybridization images are purple?
d. Digoxigenin-labeled nucleotides in the probe react with NBT/BCIP to form a purple product.

4. In situ hybridization detects:
b. Both RNA and protein

5.c. Active transport along the cytoskeleton is NOT a mechanism for control of mRNA expression by cytoplasmic localization

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