Dilution Samples: Need to make 200 uL of each dilution We are doing serial dilut

ID: 915634 • Letter: D

Question

Dilution Samples:

Need to make 200 uL of each dilution

We are doing serial dilutions:

For milk- 1:100, 1:1000, 1:2000, 1:4000 and 1:8000

For first supernatant- 1:100, 1:500, 1:1000, and 1:2000

For Whey- 1:100, 1:200, 1:400 and 1:800

For column fractions- 1:5, 1:10, 1:20, 1:40, and 1:80

And it has to be 200 uL total in volume.

I understand that for 1:100 you would use 2uL milk and 198 uL water. And then use 2uL of this and put it in 198 uL of volume for a 1:1000.

I do not understand how much to use from the 1:1000 to do 1:2000. ETC.

Ans. 0.4 microlter form 1:1000 and dilute it by 199.6 microliter.

It can be easily understood as follows :

Since if you take 2 microliter from 1:1000 and dilute it by 198 it will be 1:10000.

Now if you take 1 microliter from 1:1000 and dilute it by 199 microliter it will be 1 :5000

Now if you take 0.5 microliter from 1:1000 and dilute it by 199.5 microliter it will be 1 :2500

Now if you take 0.4 microliter from 1:1000 and dilute it by 199.6 microliter it will be 1 :2000.

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